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. 2019 Aug 15;9:11878. doi: 10.1038/s41598-019-48047-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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m-TOR dependent autophagy in WT and sacsin KO cell line. (A) Western blotting analysis of phospho-S6 (P-S6) and total S6 (S6) and LC3 autophagy markers in WT and sacsin KO cells treated for 2 h with the mitochondrial uncoupler carbonyl cyanide 4-trifluoromethoxyphenylhydrazone (FCCP 20 µM) in the absence or the presence of the PI3K inhibitor 3-methyladenine (3-MA; 10 mM). Full-length blots are presented in the Supplementary Information 2. The densitometry ratios of P-S6/S6 normalized versus β-actin (B) and of LC3-II/LC3-I normalized versus GAPDH (C) are reported, and they show m-TOR dependent regulation of autophagy in both cell lines. The data shown in this figure were reproduced independently three times. ns, not statistically significant; *p < 0.05; **p < 0.01; and ***p < 0.001.