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. 2019 Aug 16;10(9):622. doi: 10.1038/s41419-019-1847-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — THP-1 cells were treated with either 1 μg/ml LPS alone or in combination with 20 μM MKC8866 for 24 h followed by addition of 10 μM nigericin (NG) alone or in combination with 100 mM KCl for 45 min after which cells and conditioned medium were harvested. Immunoblotting was used to determine a ASC oligomerization status and b caspase-1 (pro- and cleaved p10) and IL-1β processing. c IL-1β levels were quantified by ELISA in THP-1 conditioned medium following indicated treatments (n = 3). d The percentage of THP-1 cells displaying caspase-1-like activity following the indicated treatments was assessed by the FLICA assay (n = 4). **P < 0.01 and ***P < 0.001 based on a Student’s t test. Error bars represent SD.