Table 3.

Parameter inputs to the computational models.

Parameter	Range or value	Note
Construct radii (r)	54–2142.5 μm	12
Mean organoid cell density (ρ)	2.52•1014 cells/m3	Close to in vivo density26
Single cell maximal OCR for human stem cells	2.75•10−17 mol/cell.s	44
Initial oxygen concentration	0.2 mol/m3	Maximum available oxygen concentration in water, from Henry’s law
Michaelis-Menten constant for stem cells (km1)	0.201 mol/m3	44
Oxygen diffusion in the organoid (Dorg)	1.07•10−9 m2/s	Typical in vivo value27
Oxygen diffusion constant in gel shell (DM)	10−9 m2/s	28
Oxygen diffusion constant in water at 37 °C	3•10−9 m2/s	25
Oxygen concentration on hydrogel boundary (Co)	0.2 mol/m3	45
Inlet velocity	10−3 m/s	29
Minimum viable oxygen concentration (Ccrit)	0.04 mol/m3	3