Table 2.

Binding affinities of Stl WT and dimerization mutants for trimeric and dimeric Duts

	Dutɸ11 (BLI)a			DutɸDI (MST)b
	KD (M) (10−9)	kon (M−1 s−1) (104)	koff (s−1) (10−6)	KD (M) (10−10)	Signal/noisec
StlWT	1.73	2.14 ± 0.01	37 ± 6.94	3.03	10.5
StlH73C	10.3	2.45 ± 0.01	252 ± 5.29	148	48.8
StlH188C	2.57	2.19 ± 0.01	56.3 ± 5.01	63.9	15.6
StlWT + DTTd	6.72	4.18 ± 0.08	281 ± 22.7	8.60	14.8
StlH73C + DTTd	5.22	2.19 ± 0.02	114 ± 9.4	12.7	12.4
StlH188C + DTTd	5.10	1.54 ± 0.006	78.4 ± 4.35	61.3	15.1
StlWT + Cu-Pd	11.6	2.32 ± 0.01	270 ± 5.27	4.24	17.1
StlH73C + Cu-Pd	NBDe			NBDe	0.7
StlH188C + Cu-Pd	NBDe			NBDe	3.2

^aTrimeric Dutɸ11–Stl interactions were measured by biolayer interferometry (BLI)

^bDimeric DutɸDI–Stl interactions were measured by Microscale-thermophoresis (MST)

^cSignal/noise ratio was calculated by dividing the response amplitude by the noise of the run

^dDTT and Cu-P were used at a final concentration of 1 mM

^eNot binding detected in experimental condition (K_D > 1 × 10⁻⁶ M in BLI or signal/noise <5 in MST)

Source data are provided as a Source Data file