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. 2019 Aug 9;10:666. doi: 10.3389/fgene.2019.00666

Figure 4.

Figure 4

Rate of MeHg uptake and elimination. (A) Uptake of MeHg (5 μM in food) was initiated with early L2 larvae of Canton S (CS) and Hikone R (HR) strains and carried out for 45 h. Larvae were sampled at various time points for total Hg analysis (n = 3 replicates of pooled samples of 15 larvae/time point). (B–D) Elimination of MeHg was determined in larvae previously exposed to MeHg for 40 h starting at the early L1 stage. MeHg elimination was initiated by transferring L2 larvae to MeHg-free food. (B) Larvae were sampled at various time points (10 L3 larvae/time point) for total Hg analysis. Results from one of two trials is shown (see trial 2 in Figure S2 ). Larval weights (wt) are also plotted for each time point. (C, D) Semi-log plots of Hg versus time for CS (C) and HR (D) including predicted profile of Hg concentration over time based on growth dilution alone (dashed line, gr.dil).