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. 2019 Aug 9;10:666. doi: 10.3389/fgene.2019.00666

Figure 6.

Figure 6

Correlations of eclosion rate, Hg body burden, and GSH levels in wild-derived strains of flies. (A) Eclosion rate of 10 susceptible and 10 tolerant strains of isogenic wild derived strains of flies from the DGRP panel are shown together with CS and HR. Eclosion rates are expressed as an eclosion index (see Methods) and reproduced from results in Montgomery et al. (2014). (B) Hg body burden was determined on tolerant and susceptible pupae reared on 10 µM MeHg food (n = 1 replicate of pooled samples of 10 pupae). Susceptible (median Hg = 34.5 ppm) and tolerant (median Hg = 30.7 ppm) groups were statistically different (*p = 0.0017, Mann-Whitney, non-parametric test). (C) GSH was determined in lysates of pupae (n = 1 replicate of pooled samples of 10 pupae) reared on food without (solid bars) or with (hatched bars) 10 µM MeHg. Correlation statistics were done for the relationships of eclosion index with Hg body burden [# p = 0.043, linear regression slope = −0.0328(0.0151), C.I. −0.0643 to −0.00121], eclosion index with GSH levels (linear regression, n.s., non-significant), Hg body burden with GSH levels (linear regression, n.s., non-significant).