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. 2019 Aug 9;10:666. doi: 10.3389/fgene.2019.00666

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Copyright © 2019 Rand, Vorojeikina, Peppriell, Gunderson and Prince

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rescue of MeHg-inhibited eclosion with no change in Hg body burden. (A, B) Gal4 > UAS expression pattern of drivers specific to nervous system (A, ELAV>) and muscles (B, Mef2>) in late pupal stages demonstrated in combination with UASGFP (Orientation: dorsal view, anterior to the left). (C, D) Eclosion with GCLc over-expression restricted to the nervous system (ELAV>), muscles (Mef2>) (*p < 0.05, **p < 0.001, z-test). (E, F) Hg body burden in pupae with GCLc over-expression restricted to the nervous system (ELAV>), muscles (Mef2>) (n = 3 replicates of pooled samples of 10 pupae). (G, H) Distribution of Hg (% of total body burden) in head, thorax, and abdomen with GCLc over-expression restricted to the nervous system (ELAV>, G), muscles (Mef2>, H) (n = 3 replicates of pooled samples of each body region dissected from 10 pupae, *p < 0.05, Wilcoxon-rank test).