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. 2019 Aug 9;10:880. doi: 10.3389/fphar.2019.00880

Figure 5.

Figure 5

Inhibition of Smad3 and/or JNK signaling reduces renal fibrosis and inflammation in folic acid (FA)–induced kidney injury. (A) Masson trichrome staining shows histological changes in the kidneys 4 weeks after buffer or folic acid injection with SP600125, SIS3, or combined SP600125+SIS3 treatment. (B) Confocal microscopy shows staining of α-SMA, fibronectin (FN), Col IV, and CD11b in the kidneys 4 weeks after buffer or folic acid injection with different treatments. Magnification: ×400 (A), ×600 (B and E). (C) Quantification of α-SMA, FN, and Col IV interstitial staining area. (D) The number of CD11b(+) macrophages in the kidneys 4 weeks after buffer or folic acid injection with different treatments. (E) Serum cystatin c levels in mice 4 weeks after buffer or folic acid injection with different treatments. Data are mean ± SD, (n = 6/group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.