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. 2019 Aug 9;10:888. doi: 10.3389/fphar.2019.00888

Figure 2.

Figure 2

Aldosterone dysregulates βAR signaling in vitro in fibroblasts. (A) Representative immunoblots (upper panels) and densitometric quantitative analysis (lower panel) of multiple independent experiments (n = 4) to evaluate extracellular signal-regulated kinase (ERK) 1/2 phosphorylation (pERK) as a ratio of activated ERK to total ERK (tERK). The cells were either NS or stimulated with aldosterone (Aldo, 1 µM) and/or CMPD101 (3 µM) for 12 h. After Aldo and/or CMPD101 treatment, cells were stimulated with isoproterenol (ISO, 10 µM) for 15 min. Tukey’s post hoc test. *p < 0.05 vs NS; #p < 0.05 vs ISO. (B) Dot plots showing levels of cyclic adenosine 3′,5′-monophosphate (cAMP, pmol/ml) in 3T3 fibroblasts NS or stimulated with ISO (10 µM) for 15 min. Prior ISO stimulation some groups of cells were pretreated with Aldo (1 µM) and/or CMPD101 (3 µM) for 12 h. Tukey’s post hoc test. *p < 0.05 vs NS.