Table 2.
Cryo-EM Data Collection, Refinement, and Validation Statistics
| BRISC-SHMT2 (EMDB: EMDB-0132; PDB: 6H3C) | |
|---|---|
| Data Collection and Processing | |
| Magnification | 58,140 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 45 |
| Defocus range (μm) | −0.5 to −5 |
| Pixel size (Å) | 0.86 |
| Symmetry imposed | C2 |
| Initial particle images (no.) | 332,598 |
| Final particle images (no.) | 35,595 |
| Map resolution (Å) | 3.86 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.86–15 |
| Refinement | |
| Initial model used (PDB code) | BRCA1-A (PDB: 6GVW), SHMT2 (PDB: 5V7I) |
| Model resolution (Å) | 3.86 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | N/A |
| Model composition | |
| Non-hydrogen atoms | 25,134 |
| Protein residues | 3,154 |
| Water | 2 |
| B factors (Å2) | |
| Protein | 90.25 |
| Water | 64.48 |
| RMSDs | |
| Bond lengths (Å) | 0.020 |
| Bond angles (°) | 1.93 |
| Validation | |
| MolProbity score | 1.32 |
| Clashscore | 1.88 |
| Poor rotamers (%) | 0.07 |
| Ramachandran plot | |
| Favored (%) | 94.56 |
| Allowed (%) | 4.8 |
| Disallowed (%) | 0.64 |