Skip to main content
. 2019 Aug 8;75(3):605–619.e6. doi: 10.1016/j.molcel.2019.05.026

Figure 5.

Figure 5

EXD2 Protects Replication Forks against Uncontrolled Degradation of Nascent DNA

(A) Boxplot of CldU/IdU tract ratios of HeLa WT and EXD2−/− cells (left panel) and U2OS WT and EXD2−/− cells (right panel) (5–95 percentile, n ≥ 300 tracts pooled from 3 independent experiments, Mann-Whitney).

(B) Quantification of U2OS cells or U2OS cells stably expressing GFP-EXD2 for GFP/BRCA1 PLA foci (mean ± SEM from 3 independent experiments, Mann-Whitney) and representative images. Scale bar, 10 μm.

(C) Boxplot of CldU/IdU tract ratios of HeLa WT and EXD2−/− cells untreated or pre-treated with MRE11 inhibitor Mirin (50 μM) (5–95 percentile, n ≥ 300 tracts pooled from 3 independent experiments, Mann-Whitney).

(D) Boxplot of CldU/IdU tract ratios of HeLa WT and EXD2−/− cells upon MRE11 knock-down (5–95 percentile, n ≥ 300 tracts pooled from 3 independent experiments, Mann-Whitney).