Fig. 2.
A SUMO1-specific binding site (SOB) in Arkadia mediates specificity. a Dissociation constants (KD) for the dimeric and trimeric SUMO chains listed derived from ITC measurements such as the ones shown in Supplementary Fig. 3. N.d., not determined. b Depiction of the wild-type SOB sequence and its mutant version (SOB*), in which two Val residues were mutated to Ala in an alignment with a similar motif in DPP9. c, d, Comparison of targeting efficiencies of the same substrates as in Fig. 1c by wild-type and SOB* versions of Arkadia upon their co-expression in S. cerevisiae. The analysis was carried out as described in Fig. 1c. Bars represent the means. Standard deviations (SD) are indicated by error bars. Quantifications are derived from experiments with nine independent transformants for wild-type Arkadia (same as in Fig. 1c) and three independent transformants for its SOB* mutant version. Asterisks indicate significant differences with p-values < 0.02 (one), p-values < 0.001 (three). Statistical significance was determined with unpaired and two-sided Student’s t-tests. Source data for a, c, and d are provided as a Source Data file