Histone H3 acetylation and phosphorylations. Levels of H3AcK9 (A); H3AcK9PS10 (B); H3PS10 (C); and H3PS28 (D) are presented. The experimental details were as described in Fig. 2. The levels of these components were determined by Western blotting. Values are fold change over respective sex control and are mean ± S.E.M. (n = 4 rats). a, Significant compared with control (P < 0.05); b, significant compared with the male ethanol binge group; (P < 0.05). FC, female control; FE, female ethanol binge; MC, male control; ME, male ethanol binge.