DGKα protein levels. In whole-cell extracts, DGKα protein levels in WCE were determined by Western blotting, followed by densitometric quantification. Increases in the protein levels after binge ethanol are presented as fold increases over respective sex controls. The experimental details were as described in Fig. 2. Values are mean ± S.E.M. (n = 4 rats). a, Significant compared with control (P < 0.05); b, significant compared with the male ethanol binge group; (P < 0.05). FC, female control; FE, female ethanol binge; MC, male control; ME, male ethanol binge.