Figure 2.

The probe family based upon NTA-metal coordination with their applications in protein tracking. Typical Ni-NTA-based probes were presented in (A) whilst our three generations of homemade probes were listed as (B), including Ni-NTA-AC, Ni-NTA-AF, and Ni-NTA-AB. Then the explorations for metal-associated proteomes in diverse system by using M-TRACER were presented as (C) the covalent labeling ability of M-TRACER toward intracellular proteins with UV-activation, (D) Fe-associated proteome tracked by Fe-TRACER in P. gingivalis and the protein-binding model released by X-ray crystallography, (E) Bi-associated proteome labeled by Bi-TRACER in H. pylori. (C,D) were reproduced from Jiang et al. (2018) with the permission of The Royal Society of Chemistry. (E) was reproduced from Wang et al. (2017) with the permission of The Royal Society of Chemistry.