Figure 3.

Analyses of intracellular ROS production, caspase-3 activation, and cell viability. (A) Representative confocal scanning microscope images of N2A cells showing intracellular ROS production and caspase-3 activation after transfection with vehicle, control IBs, and TDP-43 IBs. The green fluorescence arises from the CM-H₂DCFDA probe that has reacted with ROS and from FAM-FLICA^TM Caspase 3&7, respectively. (B) Quantitative analysis of the green fluorescence arising from CM-H₂DCFDA and FAM-FLICA^TM Caspase 3&7 probes. (C) MTT reduction of N2a cells transfected with vehicle, control IBs and, TDP-43 IBs and analyzed after 24 h. Error bars are S.D. *** p < 0.001 relative to cells transfected with vehicle.