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. 2019 Jul 27;20(15):3685. doi: 10.3390/ijms20153685

Figure 5.

Figure 5

TDP-43 levels in N2A cells following the treatment with UPS and ALP inhibitors and the transfection with TDP-43 aggregates. (A) Representative confocal scanning microscope images of N2A cells that were treated or not with 100 nM epoxomicin or 400 nM bafilomycin, then transfected with TDP-43 IBs and analyzed at the indicated time points after transfection. Green fluorescence indicates total TDP-43 (human and murine) detected with immunofluorescence. (B,C) Kinetic plots showing cytoplasmic (B) and nuclear (C) TDP-43 derived fluorescence, detected with Abs, against the total TDP-43 as a function of time after transfection of N2A cells with control IBs in the absence (yellow) or in the presence of a pre-treatment with epoxomicin (magenta) or bafilomycin (pink) and TDP-43 IBs in the absence (green) or in the presence of a pre-treatment with epoxomicin (blue) or bafilomycin (cyan). The line in panel (B) represents the best fits to an exponential function. Error bars are S.D. §§ p < 0.01 relative to cells without inhibitors.