Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 2;24(15):2826. doi: 10.3390/molecules24152826

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(A) Top row: The solution-phase single molecule observation limits measurement time to the diffusion time in the laser focus. Middle row: Tethering to the coverslip extends the observation time but precludes translation to live-cell studies. Bottom row: Real-time 3D single molecule tracking spectroscopy potentially enables continuous observation of freely diffusing proteins in the cellular interior. (B) An example of a simulated lifetime of a single fluorophore labelled protein diffusing through a confocal volume, emitting on the order of 10 photons, yielding a large experimental error.