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. 2019 Aug 2;24(15):2826. doi: 10.3390/molecules24152826

Figure 9.

Figure 9

(A) Schematic of tetrahedral excitation tracking setup, where four laser diode beams are combined by three beamsplitters and focused onto the sample in a tetrahedral pattern. (inset) Washers inserted between the fiber coupler (FC) and collimator (Col) are used to arrange the four laser foci after the objective. Adapted from reference [91]. Copyright 2015 Optical Society of America. (B) Schematic of TSUNAMI. A pulsed laser is split by two beamsplitters to generate four laser beams. The temporal separation between four beams is achieved by physical delay. The arrangement of the four foci after the objective is controlled by mirrors in the excitation beam path. (c) Temporal separation of the four excitation beams measured by the fluorescence of an emitter in the center of the detection volume. (D) Schematic of the tetrahedral PSF. (E) Scanning mage of 100 nm fluorescent beads in different depth. Scale bar: 2 μm. Adapted from reference [92] under Creative Commons Attribution 4.0 International License.