Figure 3.

Effects of the expression of UmHal3 in S. cerevisiae on the tolerance to diverse stress conditions. (A) Wild type BY4741 (WT) and its hal3 derivative were transformed with plasmid pWS93 (episomal, URA3 marker) and the same plasmid expressing ScHal3, full-length UmHal3, its PPCDC domain (PD) and this domain followed by the acidic C-terminal tail form ScHal3 (PD_ScCt). Cells were spotted on synthetic medium plates lacking uracil supplemented with the indicated compounds. Growth was recorded after 48 h. (B) Protein extracts from cultures of the strains described in (A) were prepared, subjected to SDS-PAGE, and proteins were transferred to membranes. The different 3xHA-tagged proteins were detected with anti-HA antibodies (indicated by arrowheads). Ponceau staining of the membranes is shown.