Hepatitis B virus surface antigen (HBsAg) reactivity of the serum samples in domestic and wild animals from Brazil and Uruguay. The dotted line corresponds to the cut-off line of the analysis, with reactive samples for the HBsAg marker shown above the dotted line and non-reactive samples for the same marker shown below the dotted line. A statistical analysis between groups was conducted in accordance with the Kruskal–Wallis test (95% confidence intervals). The Sus scrofa group corresponds to domestic pigs, wild boars, and wild pigs. The wild carnivores group corresponds to jaguar (Panthera onca), crab-eating foxes (Cerdocyon thous), maned wolves (Chrysocyon brachyurus), crab-eating raccoons (Procyon cancrivorus), and hoary foxes (Lycalopex vetulus). OD/CO = absorbance value (optical density)/cut-off ratio. p*** corresponds to p values less than 0.001.