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. 2019 Aug 2;20(15):3789. doi: 10.3390/ijms20153789

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Assessment of the membrane fraction purity. On-blot immunodetection of proteins representative of each subcellular fraction was performed for the whole-cell extracts and the membrane fractions obtained from Mel202 cells. An antibody against L1CAM was used as a control for the membrane fraction, an antibody against actin for the cytoskeletal fraction, antibody against sirtuin-6 for the nuclear fraction, and an antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for the cytosolic fraction.