Figure 2.

Concentration-dependent vasodilatation induced by Calafate berry extracts and the mimetic action of delphinidin-3-glucoside, quercetin, quercetin-3-rutinoside or acetylcholine in the rat arterial mesenteric bed. (A) Representative tracings of the hydroalcoholic extract-induced vasodilatation elicited by perfusion with 1 or 10 µg/mL; the extract was dissolved in distilled water and diluted in perfusion buffer. (B) The normalized 1 µM ACh concentration-dependent vasodilatation curves of the hydroalcoholic extract (solid circles) were compared to the main anthocyanin (delphinidin-3-glucoside, D3G open triangle) or flavonol (quercetin-3-rutinoside, Q3R solid diamond). (C) Comparison of the vasodilatation elicited by the Calafate extract, quercetin (open circles) or acetylcholine (ACh, open square). Symbols represent the mean values; bars the SEM. The number in parenthesis of panels B and C represents protocol replicates. (D) Representative tracings of the vasodilator response induced by 100 nM and 10 µM delphinidin-3-glucoside (D3G). All the experiments were made in intact mesenteric beds (with endothelium).