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(a) The transforming growth factor beta 1 (TGF-β1) mRNA and protein levels in wounds are determined by RT-PCR and western blotting, respectively (n = 6). (b) The mRNA level of Smad2, Smad3 and Smad7 is determined by RT-PCR (n = 6). # p < 0.05 and ## p < 0.01 relative to untreated control group; & p < 0.05 and && p < 0.01 relative to Blank-Gel; * p < 0.05 and ** p < 0.01 relative to CMC.
