Table 2.
Recoveries, intra-and inter-day precisions of ochratoxin A (OTA), and ochratoxin α (OTα) in different matrices (n = 6).
| Matrix | Mycotoxin | Spiked Level (ng mL−1) | Recovery (Mean ± SD, %) | Intra-Day Precision (RSD, %) | Inter-Day Precision (RSD, %) |
|---|---|---|---|---|---|
| Milk | OTA | LLOQ | 78.6 ± 4.0 | 5.0 | 10.7 |
| 1 | 95.4 ± 10.7 | 11.2 | 12.0 | ||
| 50 | 86.3 ± 5.8 | 6.7 | 11.0 | ||
| 200 | 80.1 ± 8.1 | 10.1 | 11.3 | ||
| OTα | LLOQ | 106.7 ± 5.3 | 4.9 | 12.2 | |
| 1 | 88.9 ± 9.4 | 10.6 | 11.9 | ||
| 50 | 94.6 ± 8.0 | 8.5 | 12.1 | ||
| 200 | 100.3 ± 3.5 | 3.5 | 8.6 | ||
| Urine | OTA | LLOQ | 75.3 ± 4.1 | 5.5 | 12.6 |
| 1 | 82.4 ± 5.2 | 6.3 | 11.3 | ||
| 50 | 87.4 ± 6.1 | 6.9 | 10.8 | ||
| 200 | 88.7 ± 6.0 | 6.8 | 9.9 | ||
| OTα | LLOQ | 99.3 ± 6.4 | 6.5 | 10.5 | |
| 1 | 83.8 ± 8.1 | 9.6 | 12.2 | ||
| 50 | 79.3 ± 5.8 | 7.3 | 9.0 | ||
| 200 | 84.3 ± 6.8 | 8.1 | 11.7 | ||
| Plasma | OTA | LLOQ | 87.9 ± 9.7 | 11.0 | 13.4 |
| 1 | 84.9 ± 7.3 | 8.6 | 11.3 | ||
| 50 | 83.5 ± 8.8 | 10.6 | 10.6 | ||
| 200 | 75.6 ± 4.5 | 5.9 | 10.1 | ||
| OTα | LLOQ | 114.1 ± 2.2 | 1.9 | 10.3 | |
| 1 | 87.9 ± 8.6 | 9.8 | 12.9 | ||
| 50 | 97.3 ± 4.4 | 4.5 | 6.1 | ||
| 200 | 92.6 ± 11.8 | 12.8 | 13.6 | ||
| Heart | OTA | LOQ | 112.1 ± 4.9 | 4.4 | 8.6 |
| 1 | 94.0 ± 7.5 | 8.0 | 11.9 | ||
| 50 | 91.1 ± 9.4 | 10.4 | 11.8 | ||
| 200 | 99.0 ± 3.6 | 3.7 | 8.6 | ||
| OTα | LLOQ | 101.1 ± 5.9 | 5.8 | 12.4 | |
| 1 | 87.0 ± 9.7 | 11.2 | 12.0 | ||
| 50 | 96.8 ± 9.0 | 9.3 | 11.7 | ||
| 200 | 89.2 ± 9.1 | 10.2 | 10.3 | ||
| Liver | OTA | LLOQ | 101.1 ± 6.3 | 6.3 | 10.2 |
| 1 | 91.0 ± 6.5 | 7.1 | 10.3 | ||
| 50 | 102.1 ± 6.8 | 6.7 | 11.9 | ||
| 200 | 91.9 ± 9.7 | 10.6 | 12.3 | ||
| OTα | LLOQ | 81.6 ± 6.9 | 8.4 | 11.6 | |
| 1 | 94.7 ± 5.2 | 5.5 | 9.7 | ||
| 50 | 102.8 ± 5.0 | 4.9 | 9.3 | ||
| 200 | 95.4 ± 4.3 | 4.5 | 10.8 | ||
| Spleen | OTA | LLOQ | 109.8 ± 8.0 | 7.3 | 7.9 |
| 1 | 97.8 ± 6.3 | 6.5 | 12.5 | ||
| 50 | 91.1 ± 2.1 | 2.3 | 11.4 | ||
| 200 | 96.7 ± 5.6 | 5.8 | 10.4 | ||
| OTα | LLOQ | 105.6 ± 4.3 | 4.1 | 12.1 | |
| 1 | 99.4 ± 8.9 | 8.9 | 9.7 | ||
| 50 | 93.0 ± 6.5 | 7.0 | 12.0 | ||
| 200 | 90.1 ± 6.4 | 7.1 | 10.9 | ||
| Lung | OTA | LLOQ | 99.0 ± 4.9 | 5.0 | 8.0 |
| 1 | 89.6 ± 9.1 | 10.2 | 11.6 | ||
| 50 | 89.0 ± 8.0 | 8.9 | 9.8 | ||
| 200 | 85.3 ± 5.0 | 5.8 | 10.1 | ||
| OTα | LLOQ | 107.5 ± 6.4 | 5.9 | 10.5 | |
| 1 | 97.7 ± 3.8 | 3.9 | 9.4 | ||
| 50 | 94.0 ± 5.7 | 6.0 | 11.4 | ||
| 200 | 98.0 ± 5.8 | 5.9 | 11.6 | ||
| Kidney | OTA | LLOQ | 105.2 ± 8.0 | 7.6 | 12.6 |
| 1 | 91.0 ± 5.4 | 6.0 | 11.2 | ||
| 50 | 99.5 ± 2.5 | 2.6 | 10.9 | ||
| 200 | 88.1 ± 9.1 | 10.4 | 12.8 | ||
| OTα | LLOQ | 108.9 ± 6.3 | 5.8 | 10.8 | |
| 1 | 90.9 ± 10.7 | 11.8 | 11.6 | ||
| 50 | 94.1 ± 7.8 | 8.3 | 12.9 | ||
| 200 | 87.4 ± 8.7 | 9.9 | 10.2 |
“LLOQ”: lower limit of detection.