Figure 4.

Immunofluorescence localization of MPK6 in the abscission zone (AZ) of yellow lupine flowers grown under drought conditions (C–E) and in the control AZ (B). The AZs were excised on the 48th day of development. Control plants were cultivated under optimal soil conditions (70% WHC), whereas stressed plants were subjected to drought for 2 weeks (25% WHC). The AZ region of stressed plants was highlighted by white lines (C). Images D and E present the vascular bundle area. Nuclei were stained with DAPI. The examined region of control AZ used in the immunofluorescence studies os indicated by a red square (A). Abbreviations: PROX—stem fragments below the AZ, DIST—flower pedicel fragments above the AZ, VB—vascular bundles. Scale bars: 100 µm (A, C), 40 µm (B,D,E).