Figure 3.

The mRNA expression level of Notch1 in MDA-MB-231 breast cancer cells after (histone deacetylase inhibitors) HDIs and CDDP treatment. Expression of Notch1 was analyzed by qPCR in MDA-MB-231 cells exposed to the culture medium alone (control), VPA (½ IC₅₀; IC₅₀), or SAHA (½ IC₅₀; IC₅₀) individually or in combination with CDDP (½ IC₅₀ + ½ IC₅₀, IC₅₀ + IC₅₀) for 24h. The differences between groups were evaluated using the one-way analysis of variance (ANOVA); Tukey’s post-hoc test. p < 0.05 was considered to indicate a statistically significant difference (*** p < 0.001). Results from three independent experiments (n = 9) were presented as the mean ± standard error of the mean (±S.E.M).