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. 2019 Aug 1;20(15):3763. doi: 10.3390/ijms20153763

Figure 1.

Figure 1

(A,B), Effect of vitamin B2 (riboflavin) (C,D), vitamin B6 (pyridoxine) (E,F), vitamin B9 (folic acid) (G,H), NaOH control on U937 cell proliferation. Cells were incubated with increasing doses of vitamin B for 6 days in 96 well U bottom plates and analyzed by MTT assay. Absorbance readings were taken at 540 nm to assess for cellular proliferation compared to control well (0 μg/mL). Significance was established at p, 0.05, two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test and marked with asterisk (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Cells were viewed under an IX81 Olympus microscope at 4x magnification and photos taken at each concentration and control NaOH on day 6 of culture.