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. 2019 Jul 29;20(15):3702. doi: 10.3390/ijms20153702

Figure 2.

Figure 2

Insulin decreases ROS formation in cortical neuron exposed to hydrogen peroxide. The results of one typical experiment (from five replicates) are given as means ± SEM of 4–6 parallel determinations. Brain cortical neurons were incubated with 1 µM or 100 nM insulin for 1 h (A), 5 h (B) or 20 h (C), then the cells were exposed to 100 µM hydrogen peroxide. ROS accumulation was estimated using dichlorofluorescin diacetate. The differences are significant according to one-way ANOVA as compared: *—to the control values, p < 0.01; x and xx—to the effect of hydrogen peroxide alone, x p < 0.05, xx p < 0.01.