Figure 9.

Insulin significantly diminishes the level of pAMPKalpha (Thr¹⁷²) markedly elevated in cortical neurons by their exposure to hydrogen peroxide. A—immunoblots obtained in one typical experiment (from seven replicates) are shown. B—results of seven experiments are presented as means ± SEM. Cortical neurons were incubated with insulin (or without it) for 1 h and then exposed to 100 µM hydrogen peroxide. The differences of insulin and hydrogen peroxide effects as compared to the effect of hydrogen peroxide alone on the level of pAMPKalpha (Thr¹⁷²) in cortical neurons are significant according to Student’s paired t test: * and **—the effect of preincubation with 100 nM insulin is significant, * p < 0.05, ** p < 0.02, ^x and ^xx—the effect of preincubation with 1 µM insulin is significant, ^x p < 0.05, ^xx p < 0.02.