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. 2019 Aug 1;20(15):3772. doi: 10.3390/ijms20153772

Figure 5.

Figure 5

Expression of auxin-relative gene in tobacco under different Se and Cd concentration supply conditions. (A–C) Expression of three members (YUCCA6, 8, 9) of the tobacco YUCCAs family genes in shoots under different Se and Cd concentration supply conditions.; (D–F) Expression of three members (PIN1a, 1c, 4) of the tobacco PINs family genes in roots under different Se and Cd concentration supply conditions. 14-days-old seedlings (DR5::GUS transgenic tobacco) were grown in pot with sand under different Se (0, 10, 50 μM) and Cd (0, 20, 50 μM) concentrations for 21 days. The tobacco housekeeping gene L25 was used as an internal control. The relative expression levels are shown compared with the expression under Cd0 and Se0 (Cd0 + Se0) conditions as 1 expression. Se0: no Se; Se10: Se, 10 μM; Se50: Se, 50 μM; Cd0: no Cd; Cd20: Cd, 20 μM; Cd50: Cd, 50 μM. Shown are mean ± SD from five biological replicates. Different letters indicate significant differences (p < 0.05).