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. 2019 Jul 29;20(15):3700. doi: 10.3390/ijms20153700

Figure 4.

Figure 4

Combination treatment with CHK1 and ATM inhibitors induces NB-39-nu cell death. (A–C) NB-39-nu cells were treated with DMSO (NT), 0.175 μM PF-477736 (CHK1i), 10 μM Ku55933 (ATMi), or 0.175 μM PF-477736 plus 10 μM Ku55933 (CHK1i + ATMi). Cells were then harvested and analyzed for (A) alamarBlue cell viability assay, (B) protein expression by immunoblotting at 7 days after indicated treatments, or (C) Giemsa staining and microscopy at 6 days after indicated treatments. In (A), the graphs were plotted using the values from four independent experiments. Data are presented as the mean ± SD. * p < 0.05. cl-CASP3, cleaved caspase-3; PARP, poly (ADP-ribose) polymerase. In (B), Representative numbers were normalized to the intensity of the indicated bands. In C, black arrows indicate the examples of cell shrinkage with disruption of cell–cell contacts.