Figure 7.

Schematic representation of experimental design. After seed germination, the seedlings were grown for 8 weeks in the Roullier high-throughput plant phenotyping platform. Foliar application of Ca²⁺ was supplied twice at BBCH14 and BBCH18, each time with a dose of 5 L ha⁻¹ corresponding to 540 g Ca ha⁻¹. Drought stress (30% field capacity) was imposed 2 days after the second application of Ca²⁺ for a duration of 3 weeks, while control plants were kept in 90% of field capacity. From BBCH14 till end of the experiment, image-based phenotyping was applied every 4 days. All plants were harvested at 60 days after sowing for different physiological, biochemical, and molecular analyses.