Figure 1.

Inhibition of cell proliferation by licochalcone A (LCA) in human bladder cancer cells. T24, 5637, Chang, and HaCat cells were evenly distributed in 96-well plates and treated with LCA (10~60 μM) for 48 h. As described in Materials and Methods, the ability of LCA to inhibit cell proliferation was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetra-zolium bromide (MTT) assay, and cell viability values were expressed relative to those wells where no LCA was added (100% control value). Results represent the mean ± standard deviation (SD) of at least three independent experiments (** p < 0.001 and *** p < 0.0001 compared to control).