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. 2019 Jul 24;24(15):2699. doi: 10.3390/molecules24152699

Figure 4.

Figure 4

Effects of ABSP and ABSSP on mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) in LPS-stimulated RAW264.7 macrophage cells. RAW264.7 cells were pretreated with ABSP, ABSSP (20 and 100 μg/mL), and BAY11-7082 (BAY, 5 μM) for 2 h, and then stimulated with LPS (0.5 μg/mL) for 24 h. The mRNA levels of iNOS (A) and COX2 (B) were measured by qRT-PCR; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. Cell lysates were immunoblotted with anti-iNOS and anti-COX2 antibodies. GAPDH was used as a loading control. Results from representative experiments are shown (C), and the quantitative results of iNOS (D) and COX2 (E) are depicted. Data are expressed as mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with LPS.2.4. ABSP and ABSSP decreased the mRNA expression of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in LPS-activated RAW264.7 cells.