Figure 3.

Principal component analysis score plot of all variables of the tested different horseradish (Armoracia rusticana) hairy root clones, including glucosinolate content, isothiocyanate content as well as enzyme activities/patterns. (a) Principal component analysis score plots showing different hairy root clones (HRCs) in the PC1—PC2 plane, separated by their features. The lines of different origins are shown with different colors: red—petiole, blue—leaf blade. (b) Contribution of features (loading values) to score values of horseradish hairy root cultures, in principle component 1 (PC1). Feature abbreviations: HRP_pg—horseradish peroxidase activity with pyrogallol as substrate (mmol pyrogallol min⁻¹ mg⁻¹ protein); HRP_gu—horseradish peroxidase activity with guaiacol as substrate (mmol guaiacol min⁻¹ mg⁻¹ protein); Myr—myrosinase activity; ProtContent—protein content (mg protein mg⁻¹ fresh weight); MeSPITC—3-(methylthio)propyl isothiocyanate (abundance); PEITC—2-phenylethyl isothiocyanate (µg mg⁻¹ DW); AITC—allyl isothiocyanate (µg mg⁻¹ DW); DWpc—dry weight %; DGI—daily growth index; PECN—3-phenylpropionitrile (abundance); I3ACN—indole-3-acetonitrile (abundance); HRP_pg_AUC—horseradish peroxidase content with pyrogallol as substrate (abundance); HRP_gu_AUC—horseradish peroxidase content with guaiacol as substrate (abundance); ARAB—glucoarabishirsutain (abundance); SIN—sinigrin (µg mg⁻¹ DW); GIB—glucoibarin (abundance); BRASS—glucobrassicin (abundance); NEO—neoglucobrassicin (abundance); IBER—glucoiberverin (abundance); GLN—gluconasturtiin (µg mg⁻¹ DW). (c) Contribution of features (loading values) to score values of horseradish hairy root cultures, in principle component 2 (PC2). Feature abbreviations: same as in Figure 3b.