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(a) Treatment imaging flow cell (FC) under CLSM for time lapse experiments; (b) time lapse CLSM of ethanol action performed on E. coli biofilm grown on low density polyethylene surfaces functionalized with p-aminosalicylic acid. The fluorescence loss from stained E. coli cells is used to monitor real-time loss in cell viability during the biocide action. Under ethanol treatment, biofilm displayed a total loss in fluorescent intensity in 5 min.
