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. 2019 Jul 30;20(15):3719. doi: 10.3390/ijms20153719

Figure 1.

Figure 1

Schematic diagram of the ribozyme-flanked artificial sgRNA (RGR). (A) An RGR construction (P: Any promoter; T: Terminator; Ribozyme: Self-cleaving ribozyme; and sgRNA: Single guide RNA); (B) Transcription of the sgRNA by RNA polymerase II (Pol II) causes changes in the primary transcript, including 5′-end capping and 3′-end poly A tail; (C) Transcription of the sgRNA by RNA polymerase III (Pol III) causes changes in the primary transcript, including 3′-end poly U tail; and (D) Ribozymes cleave the 5′-end and 3′-end modifications and produce the unchanged sgRNAs.