Figure 3.

Effects of EA-100C red on mitochondrial membrane potential. Flow cytometric analysis of mitochondrial membrane potential by using Mitotracker Red, a dye which localizes in mitochondria and changes according to mitochondrial membrane potential variations due to oxidative stress. U87MG (A) and LN229 (B) cells were treated with EA-100C red for 72 h and H₂O₂ for 1 h (left panels). CTR+ stained untreated cells, CTR- unstained untreated cells. The % MFIs of control were calculated as described in ‘Materials and Methods’ and represented as columns (right panels). The experiments were performed at least three times and the results were always similar. Bars, SDs. ** p ≤ 0.001.