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Optical micrographs showing P4HB spherulites crystallized at 47 °C for (a) 100 min, (b) 150 min, (c) 180 min, (d) 250 min, (e) 350 min and (f) 350 min. In the case of (f), the micrograph has been taken with a red tint plate. Comparison of micrographs taken after 47 min (g) and 80 min (h) for the crystallization performed at 46 °C. The dashed red circle points out the region where the apparition of a new spherulite is clear.
