Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 31;20(15):3752. doi: 10.3390/ijms20153752

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Total polyunsaturated fatty acid (PUFA) content of rat brain endothelial cells (RBE4) cells and of purified phosphatidylcholine (PC) after oxygen and glucose deprivation and restoration (OGD/OgR) treatment for 1 and 24 h. Cells, after OGD/ogR treatment, were collected and lipids were extracted by different mixture of methanol/chloroform. Cell FAs (fatty acids) were measured as FA methyl esters (FAME) by a GC (Agilent Technologies 6850 series II, Santa Clara, CA, US) equipped with flame ionization detector. FAME were quantified using the chromatographic peak area according to the internal standard (IS) method. Histograms represent the most relevant results of whole cell (A), and PC (B) FA composition. The data are normalized to cellular protein content and expressed as mean ± S.E. from three independent experiments, * p < 0.05, ** p < 0.01 vs. control.