Table 1.
Effects of ASC-Conditioned Medium/Secretome on Wound Healing: In vitro studies.
| ASC Source | Model | Effect | Reference |
|---|---|---|---|
| Human lipoaspirates from healthy females. | HDF | Increased cell proliferation and migration. Higher type I collagen secretion. | [56] |
| Human lipoaspirates from female patients (18–35 years old). | HDF | Increased cell proliferation and migration. | [57] |
| Human adipose tissue from donors undergoing abdominoplasty. | HDF | No effect. | [58] |
| HUVEC | No effect. | ||
| Human keratinocytes | Reduction of keratinocyte proliferation. | ||
| Human abdominal adipose tissue and lipoaspirates from patients undergoing panniculectomy or abdominoplasty. | 3-D skin cultures of primary human keratinocytes | Acceleration of wound closure. | [59] |
| Commercial human ASCs. | HUVEC | Increased cell proliferation, migration, and invasion ability. | [60] |
| HaCaT | Increased cell proliferation, migration, and VEGF secretion. | ||
| Commercial human ASCs of normal donors (25–45 years old) undergoing elective surgery. | HDF | Increased cell migration. | [61] |
| Adipose tissue of breast cancer patients. | HDF | Increased cell proliferation, migration, and invasion ability. | [46] |
| HEKa | |||
| HUVEC | |||
| Human sub-cutaneous adipose tissue from male patient (51-year-old) undergoing skin-graft surgery. | HDF | Increased cell proliferation and migration. | [62] |
| HaCaT | |||
| Sub-cutaneous adipose tissue (average age of patients 47.2 ± 10.8) or sub-cutaneous lipoma (average age of patients 41.8 ± 7.1). | RAW 264.7 | Functional cell activation and M2 phenotype polarization. | [64] |
| L929 Fibroblasts | Acceleration of scratched monostrate closure, increased cell migration. |
HDF: human dermal fibroblast; HUVEC: human umbilical vein endothelia cell; ASCs: adipose tissue-derived stem cells; HaCaT: spontaneously immortalized human keratinocyte cell line; VEGF: vascular endothelial growth factor; HEKa: human epidermal keratinocyte, adult.