Figure 4.

Myeloperoxidase (MPO) released from PMA-stimulated neutrophils was enhanced by oxLDL treatment. HL-60-derived neutrophils were stimulated with 50 nM PMA for 30 min following 20 μg/mL native LDL or oxLDL for additional 2 h. (A,C) Western blot analysis of culture media of the cells to detect MPO and apolipoprotein B-100 (apoB) following native PAGE on a 3–20% gradient gel. (B,D) Western blot analysis of MPO in cell lysates of HL-60-derived neutrophils following 12% gel SDS-PAGE. Quantitative data presented in the bar graph are the mean ± SD of three experiments. Asterisks indicate statistical significance against the condition “PBS without PMA”; ^***p < 0.001. Sharps indicate statistical significance against the condition “PBS with PMA”; ^##p < 0.005.