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. 2019 Jul 24;20(15):3613. doi: 10.3390/ijms20153613

Table 1.

Primer sets for Real Time-PCR.

Gene Forward (5′ to 3′) Reverse (5′ to 3′)
Osteoblast Differentiation Makers
runx2a GACGGTGGTGACGGTAATGG TGCGGTGGGTTCGTGAATA
runx2b CGGCTCCTACCAGTTCTCCA CCATCTCCCTCCACTCCTCC
sp7 (osterix) GGCTATGCTAACTGCGACCTG GCTTTCATTGCGTCCGTTTT
col1a1a GAATAAGCAGGTGGAGTCT GAAGCAACTGAACGACATT
col1a1b GGTGCTATGCTGTGATTCT GATTCTCGCTAAGTGTCCAT
alp CAAGAACTCAACAAGAAC TGAGCATTGGTGTTATAC
osteopontin GCCTCCATCATCATCGTA AATCACCAAGCACCAGTA
phex GAGAATGAATGGATGGATGA TTGATGTCTTCGTTAATATAGGT
Osteoclast Differentiation Markers
ctsk GGACTCAATCACTATCACT AGAACAAGACATCTAAGACA
rank GCACGGTTATTGTTGTTA TATTCAGAGGTGGTGTTAT
acp5b GCTGCTGCTAACAAACAAT GACCAACCACGATGACAA