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. 2019 Aug 9;10:876. doi: 10.3389/fphar.2019.00876

Figure 2.

Figure 2

Inhibition of CYP4A with HET0016 reduces myocardial apoptosis induced by AGEs in mice and cardiomyocytes. (A) Hematoxylin and eosin (HE) staining and TUNEL staining of mouse heart tissues. Scale bar = 50 μm. (B) Relative apoptosis rates are represented as TUNEL-positive cells/DAPI-positive cells, n = 6. (C–G) Protein expression of p-Akt, p-JNK, Bcl-xL, and c-caspase-3. n = 3. (H) Cell viability of rat cardiomyocytes detected with cell counting kit 8. n = 6. (I) Apoptosis of rat cardiomyocytes via TUNEL staining. (J) Relative apoptosis rates are represented as TUNEL-positive cells/DAPI-positive cells, n = 3. Cardiomyocytes were isolated and cultured from ventricles of neonatal rats. Scale bar = 10 μm. AGEs solution was administered intragastrically to C57BL/6 mice for 60 days, while the specific inhibitor of CYP4A, HET0016, was given from the 47th day via intraperitoneal injection. Con, control. Compared with the Con group, +p < 0.05, ++p < 0.01, +++p < 0.001; compared with the AGEs group, *p < 0.05, **p < 0.01 (one-way ANOVA with Tukey post hoc).