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. 2019 Aug 9;10:876. doi: 10.3389/fphar.2019.00876

Figure 5.

Figure 5

Inhibition of CYP4A by siRNAs protects H9c2 cells from AGEs-induced oxidative stress. (A) Protein expression of CYP4A. (B) Protein expression of NOX2. (C) Level of ROS generation. (D) A summary of flow cytometry analyses of cells stained with DCFH-DA. n = 3. siCon: siControl. siRNAs: siRNAs against CYP4a1, CYP4a2, and CYP4a3. H9c2 cells at a density of 1 × 105 cells per well were seeded on six-well plates and transfected at 70–80% confluence with siRNAs against rat CYP4a1, CYP4a2, CYP4a3, or nonbinding control siRNA. Transfection was performed with Lipofectamine 2000. After 24 h of Cyp4a siRNAs transfection, cells were treated with 10 μM AGEs or BSA for 24 h. Compared with the siCon group, ++p < 0.01; compared with the AGEs+siCon group, *p < 0.05, **p < 0.01 (one-way ANOVA with Tukey post hoc).