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. 2019 Aug 9;10:986. doi: 10.3389/fphys.2019.00986

FIGURE 4.

FIGURE 4

Quantification of mitochondria FA transporters expression, FA oxidation and energy production. qRT-PCR analysis of the mitochondria transporters CPT1a (A) and CPT1b (B). qRT-PCR expression analysis of enzymes involved in mitochondrial FAO: UCP3 (C), MCAD (D) and LCAD (E). Target gene expressions are measured by qRT-PCR analysis and are expressed as relative quantity on sham group. All measurements are referred to 24 and 96 h after surgery. Analysis of β-oxidation and energy production: β-ox (F), ETC (G) and ATP content (H). These measurements are referred to 24 h after surgery. All data are expressed as mean ± SD. Statistical significance refers to I/R and SLN/myr treated I/R groups as compared to sham animals. The comparison between I/R and SLN/myr treated I/R groups is indicated by the connecting line (*p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ****p < 0.0001). Sham animals: gray bar; I/R group: white bar; SLN/myr treated I/R group: black bar.