FIG 3.

In vivo Galleria mellonella model. The larva survival rates of larvae infected with NIPH 2061 cells (K2 capsule type) (10⁶ CFU inoculum) were measured by injecting K2 depolymerase-pretreated NIPH 2061 cells for 2 h (A) or K2 depolymerase 30 min after NIPH 2061 bacterial inoculation (B). Three different enzyme doses of 0.25 μg, 0.5 μg, and 3 μg were used. For clarity, control groups (HEPES-treated and enzyme-treated larvae) resulted in 100% survival rate and were omitted from the figures. Significance was determined by Mantel-Cox test (*, P < 0.05).