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. 2019 Aug 14;85(17):e01076-19. doi: 10.1128/AEM.01076-19

FIG 2.

FIG 2

Dac production in the recombinant B. subtilis WB600 using different promoters. (a) Construction of recombinant plasmid p43NMK-YncM-Dac using p43NMK vector with P43 promoter. (b) The extracellular Dac activities of the recombinant strains using different promoters. (c) SDS-PAGE analysis of extracellular Dac of the recombinant strains. M, protein marker; 1, B. subtilis WB600 strains expressing Dac using HpaII promoter; 2, B. subtilis WB600 strains expressing Dac using P43 promoter. The recombinant strains were grown on fermentation medium (see Materials and Methods) containing 10 mg/liter of kanamycin in 500-ml flask and incubated at 37°C and 220 rpm for 48 h. Error bars indicate the average values from three parallel experiments.