TABLE 4.
Strain or plasmid | Descriptiona | Source or reference |
---|---|---|
Strains | ||
E. coli JM109 | Cloning strain | Takara, Japan |
E. coli BL21(DE3) | Expression strain | Invitrogen, CA |
B. subtilis WB600 | Deficient in NprE AprE Epr Bpr Mpr NprB | Lab stock |
WB-MA-DS | B. subtilis WB600 containing pMA0911NS | This work |
WB-MA-J | B. subtilis WB600 containing pMA0911J | This work |
WB-MA-C | B. subtilis WB600 containing pMA0911C | This work |
WB-MA-M | B. subtilis WB600 containing pMA0911M | This work |
WB-MA-Z | B. subtilis WB600 containing pMA0911Z | This work |
WB-MA-O | B. subtilis WB600 containing pMA0911O | This work |
WB-MA-A | B. subtilis WB600 containing pMA0911A | This work |
WB-MA-F | B. subtilis WB600 containing pMA0911F | This work |
WB-MA-G | B. subtilis WB600 containing pMA0911G | This work |
WB-MA-L | B. subtilis WB600 containing pMA0911L | This work |
WB-MA-H | B. subtilis WB600 containing pMA0911H | This work |
WB-MA-E | B. subtilis WB600 containing pMA0911E | This work |
WB-MA-I | B. subtilis WB600 containing pMA0911I | This work |
WB-NMK/Dac | B. subtilis WB600 containing pP43NMK-YncM-Dac | This work |
WB-NMKmut/Dac | B. subtilis WB600 containing pP43NMKmut-YncM-Dac | This work |
Plasmids | ||
pET-28a-Dac | pET-28a(+) containing Dac gene | Lab stock |
pMA0911 | pMA5 derivative, PHpaII, Kanr, Ampr | Lab stock |
pP43NMK | PHpaII, P43, Kanr, Ampr | 31 |
pMA0911J | pMA0911 derivative, Dac gene, YncM | This work |
pMA0911C | pMA0911 derivative, Dac gene, Bpr | This work |
pMA0911M | pMA0911 derivative, Dac gene, YwbN | This work |
pMA0911Z | pMA0911 derivative, Dac gene, AnsZ | This work |
pMA0911O | pMA0911 derivative, Dac gene, YvgO | This work |
pMA0911A | pMA0911 derivative, Dac gene, AmyE | This work |
pMA0911F | pMA0911 derivative, Dac gene, OppA | This work |
pMA0911G | pMA0911 derivative, Dac gene, Vpr | This work |
pMA0911L | pMA0911 derivative, Dac gene, LipA | This work |
pMA0911H | pMA0911 derivative, Dac gene, WapA | This work |
pMA0911E | pMA0911 derivative, Dac gene, Epr | This work |
pMA0911I | pMA0911 derivative, Dac gene, YclQ | This work |
pMA0911NS | pMA0911 derivative, Dac gene, without signal peptide | This work |
pP43NMK-YncM-Dac | pP43NMK containing Dac gene, YncM | This work |
pP43NMKmut-YncM-Dac | pP43NMK/Dac derivative, 5′ UTR mutation | This work |
pP43NMKmut-YncM-Dac-R157T | pP43NMK/Dac derivative, 5′ UTR mutation, R157T | This work |
Ampr, ampicillin resistance; Kanr, kanamycin resistance.