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. 2019 Aug 14;85(17):e01076-19. doi: 10.1128/AEM.01076-19

TABLE 4.

Strains and plasmids used in this study

Strain or plasmid Descriptiona Source or reference
Strains
    E. coli JM109 Cloning strain Takara, Japan
    E. coli BL21(DE3) Expression strain Invitrogen, CA
    B. subtilis WB600 Deficient in NprE AprE Epr Bpr Mpr NprB Lab stock
        WB-MA-DS B. subtilis WB600 containing pMA0911NS This work
        WB-MA-J B. subtilis WB600 containing pMA0911J This work
        WB-MA-C B. subtilis WB600 containing pMA0911C This work
        WB-MA-M B. subtilis WB600 containing pMA0911M This work
        WB-MA-Z B. subtilis WB600 containing pMA0911Z This work
        WB-MA-O B. subtilis WB600 containing pMA0911O This work
        WB-MA-A B. subtilis WB600 containing pMA0911A This work
        WB-MA-F B. subtilis WB600 containing pMA0911F This work
        WB-MA-G B. subtilis WB600 containing pMA0911G This work
        WB-MA-L B. subtilis WB600 containing pMA0911L This work
        WB-MA-H B. subtilis WB600 containing pMA0911H This work
        WB-MA-E B. subtilis WB600 containing pMA0911E This work
        WB-MA-I B. subtilis WB600 containing pMA0911I This work
        WB-NMK/Dac B. subtilis WB600 containing pP43NMK-YncM-Dac This work
        WB-NMKmut/Dac B. subtilis WB600 containing pP43NMKmut-YncM-Dac This work
Plasmids
    pET-28a-Dac pET-28a(+) containing Dac gene Lab stock
    pMA0911 pMA5 derivative, PHpaII, Kanr, Ampr Lab stock
    pP43NMK PHpaII, P43, Kanr, Ampr 31
    pMA0911J pMA0911 derivative, Dac gene, YncM This work
    pMA0911C pMA0911 derivative, Dac gene, Bpr This work
    pMA0911M pMA0911 derivative, Dac gene, YwbN This work
    pMA0911Z pMA0911 derivative, Dac gene, AnsZ This work
    pMA0911O pMA0911 derivative, Dac gene, YvgO This work
    pMA0911A pMA0911 derivative, Dac gene, AmyE This work
    pMA0911F pMA0911 derivative, Dac gene, OppA This work
    pMA0911G pMA0911 derivative, Dac gene, Vpr This work
    pMA0911L pMA0911 derivative, Dac gene, LipA This work
    pMA0911H pMA0911 derivative, Dac gene, WapA This work
    pMA0911E pMA0911 derivative, Dac gene, Epr This work
    pMA0911I pMA0911 derivative, Dac gene, YclQ This work
    pMA0911NS pMA0911 derivative, Dac gene, without signal peptide This work
    pP43NMK-YncM-Dac pP43NMK containing Dac gene, YncM This work
    pP43NMKmut-YncM-Dac pP43NMK/Dac derivative, 5′ UTR mutation This work
    pP43NMKmut-YncM-Dac-R157T pP43NMK/Dac derivative, 5′ UTR mutation, R157T This work
a

Ampr, ampicillin resistance; Kanr, kanamycin resistance.